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/** |
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$Id$ |
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Copyright (C) 2009 Nicklas Nordborg |
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This file is part of BASE - BioArray Software Environment. |
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Available at http://base.thep.lu.se/ |
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BASE is free software; you can redistribute it and/or |
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modify it under the terms of the GNU General Public License |
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as published by the Free Software Foundation; either version 3 |
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of the License, or (at your option) any later version. |
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|
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BASE is distributed in the hope that it will be useful, |
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but WITHOUT ANY WARRANTY; without even the implied warranty of |
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MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the |
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GNU General Public License for more details. |
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You should have received a copy of the GNU General Public License |
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along with BASE. If not, see <http://www.gnu.org/licenses/>. |
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*/ |
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package net.sf.basedb.util.importer.spotdata; |
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import java.util.List; |
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import net.sf.basedb.util.basefile.BaseFileParser; |
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import net.sf.basedb.util.parser.FlatFileParser; |
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import net.sf.basedb.util.parser.FlatFileParser.Data; |
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/** |
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Spot intensity parser implementation for 2-channel data |
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that looks for M/A values in the spot data and converts them to ch1/ch2 |
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intensity values. The required assay fields are: |
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l2ratio1_2 (M) and l10intgmean1_2 (A). |
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<p> |
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NOTE! The column names for the M/A values can be redefined by |
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calling {@link BaseFileParser#setRedefinedColumnName(String, String, String)}. |
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@author Nicklas |
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@version 2.14 |
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@base.modified $Date$ |
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*/ |
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public class MAParser |
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implements SpotIntensityParser |
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{ |
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private int mIndex; |
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private int aIndex; |
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private static final String mColumn = "l2ratio1_2"; |
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private static final String aColumn = "l10intgmean1_2"; |
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/** |
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Creates a new MA-parser. |
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*/ |
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public MAParser() |
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{} |
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|
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/* |
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From the SpotIntensityParser interface |
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-------------------------------------- |
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*/ |
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@Override |
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public boolean hasRequiredAssayFields(BaseFileParser parser, FlatFileParser ffp, |
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List<String> assayFields) |
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{ |
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int mIndex = assayFields.indexOf(parser.getRedefinedColumnName("spots", mColumn)); |
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int aIndex = assayFields.indexOf(parser.getRedefinedColumnName("spots", aColumn)); |
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return mIndex >= 0 && aIndex >= 0; |
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} |
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@Override |
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public void beginSection(BaseFileParser parser, FlatFileParser ffp, |
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List<String> assayFields) |
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{ |
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mIndex = assayFields.indexOf(parser.getRedefinedColumnName("spots", mColumn)); |
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aIndex = assayFields.indexOf(parser.getRedefinedColumnName("spots", aColumn)); |
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} |
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|
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@Override |
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public boolean setIntensities(Data data, float[] intensities, int firstIndex) |
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{ |
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Float m = data.getFloat(mIndex + firstIndex, null, true); |
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if (m == null) return false; |
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Float a = data.getFloat(aIndex + firstIndex, null, true); |
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if (a == null) return false; |
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// int2 = 10^a / 2^(0.5*m) |
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// int1 = int2 * 2^m |
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intensities[1] = (float)(Math.pow(10, a) / Math.pow(2, 0.5 * m)); |
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intensities[0] = (float)(intensities[1] * Math.pow(2, m)); |
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return true; |
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} |
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// --------------------------------------- |
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} |