Anna Isinger-Ekstrand, Jan Johansson, Mattias Ohlsson, Princy Francis, Johan Staaf, Mats Jönsson, Åke Borg and Mef Nilbert
Genetic profiles of gastroesophageal cancer: combined analysis using expression array and tiling array-comparative genomic hybridization
Cancer Genetics and Cytogenetics 200, 120-126 (2010)
Abstract:
We aimed to characterize the genomic profiles
of adenocarcinomas in the gastroesophageal
junction in relation to cancers in the
esophagus and the stomach. Profiles of
gains/losses as well as gene expression
profiles were obtained from 27
gastroesophageal adenocarcinomas by means of
32k high-resolution array-based comparative
genomic hybridization and 27k oligo gene
expression arrays, and putative target genes
were validated in an extended
series. Adenocarcinomas in the distal
esophagus and the gastroesophageal junction
showed strong similarities with the most
common gains at 20q13, 8q24, 1q21-23, 5p15,
13q34, and 12q13, whereas different profiles
with gains at 5p15, 7p22, 2q35, and 13q34
characterized gastric cancers. CDK6 and EGFR
were identified as putative target genes in
cancers of the esophagus and the
gastroesophageal junction, with upregulation
in one quarter of the tumors. Gains/losses and
gene expression profiles show strong
similarity between cancers in the distal
esophagus and the gastroesophageal junction
with frequent upregulation of CDK6 and EGFR,
whereas gastric cancer displays distinct
genetic changes. These data suggest that
molecular diagnostics and targeted therapies
can be applied to adenocarcinomas of the
distal esophagus and gastroesophageal junction
alike.
LU TP 07-01